Heterogeneity was tested using the Q test with p<0.1 considered as statistical heterogeneity. The burden of chronic HBV infection is also unevenly distributed among the various subpopulations in the adult group and remains high per the WHO’s level of categorization. We would like to thank Editage (For more information about PLOS Subject Areas, click The National Identification Authority’s mop-up for the Ghana Card registration in the Greater Accra Region will now begin on August 24, 2020. However, in this study the Northern region consists of Northeast, Savannah, and Northern regions; Brong-Ahafo consists of Bono, Bono East, and Ahafo regions; and the Western region consists of Western and Western North regions.Meta-analysis with a random effect model was used to pool the prevalence from different homogeneous studies. Chronic hepatitis B (HBV) remains a significant public health problem in Ghana and past reviews conducted could not calculate a nationwide prevalence of the disease due to lack of primary research for some regions of the country. Derived from total population. Due to the limited number of studies included in each meta-analysis, we were not able to explore the potential of publication bias [This systematic review and meta-analysis indicated that the prevalence of HBV infection between 2015 and 2019 in Ghana was 0.55%, 14.30%, and 8.36% among pre-school children, adolescents, and adults, respectively. The region also has the largest gap between the percentage of people living with HIV who know their status and the percentage of people with HIV who know their positive status and are on treatment. The degree of heterogeneity was measured with IThe study did not require ethical approval as it used data retrieved from published studies that are already in the public domain.The reported prevalence among the 21 studies in the systematic review ranged from 0.05% to 54.20% (Two studies (528 participants in total) reported HBV infection prevalence among pre-school children, at 0.05% and 1.90%. World Population Prospects: 2019 Revision, ( 2 ) Census reports and other statistical publications from national statistical offices, ( 3 ) Eurostat: Demographic Statistics, ( 4 ) United Nations Statistical Division. The HBV prevalence ranged from 2.45% in the Northern region to 16.70% in the Western region. Hence, the prevalence is more representative of the adult, urban population. The prevalence determined in this study was therefore based on subpopulations and hence cannot be said to fully represent the general Ghanaian population. Addressing the issue will require an integrated public health strategy and rethinking of the implementation gaps in the current HBV infection control program. Prevalence was lower in the north than in the southern part of the country. This will help propel the country towards eliminating the disease by 2030.Hepatitis B virus (HBV) infection can lead to severe disease and death, affecting people worldwide. The Ghana Statistical Service (GSS) Population and Housing Census (PHC) recruitment was announced in a publication in the daily graphic dated 15th October 2019. Any discrepancies were resolved by referring to the original publications.The Newcastle-Ottawa scale (NOS) for cross-sectional studies quality assessment tool was adopted and used to assess the quality of each study [HBV infections were measured with prevalence and 95% confidence interval (CI). This systematic review and meta-analysis indicated that the prevalence of HBV infection between 2015 and 2019 in Ghana was 0.55%, 14.30%, and 8.36% among pre-school children, adolescents, and adults, respectively. Our result was lower than the 7.70% and 9.70% reported in Burkina Faso [Among pregnant women, HBV prevalence remains high (7.44%), as reported in other parts of the sub-region [Additionally, this study revealed a very high HBV prevalence of 14.40% among barbers and long-distance drivers. These were children who have been vaccinated against HBV with the pentavalent vaccine. Population source: ( 1 ) United Nations Population Division. Transfusion safety of blood and blood products in Ghana should be a major concern as about one in every ten blood donors is infected with HBV. Several factors are implicated in the observed high HBV prevalence in Ghana. Furthermore, most of the studies (13/21, 62%) were conducted in urban settings and in adult populations (18/21, 86%).